Transcription sans TBP

Targeting Transcription Through Inhibition of TBP

Transcriptional regulation by RNA polymerase II (RNA Pol II) is a highly regulated process involving the action of multiple transcription factors that collectively regulate synthesis of messenger RNA (mRNA). These complexes sit atop core promoter regions of DNA composed of sequence motifs that mark the starting point of transcription [1]. Studies have shown that the transcriptional machinery is...

Duality of TBP, the universal transcription factor.

TBP, a universal eukaryotic transcription factor?

Transcription factor IIA derepresses TATA-binding protein (TBP)-associated factor inhibition of TBP-DNA binding.

The interaction of the general transcription factor (TF) IIA with TFIID is required for transcription activation in vitro. TFIID consists of the TATA-binding protein (TBP) and TBP associated factors (TAFIIs). TFIIA binds directly to TBP and stabilizes its interaction with TATA-containing DNA. In this work, we present evidence that TAFIIs inhibit TBP-DNA and TBP-TFIIA binding, and that TFIIA sti...

TBP mutants defective in activated transcription in vivo.

The TATA box binding protein (TBP) plays a central and essential role in transcription initiation. At TATA box-containing genes transcribed by RNA polymerase II, TBP binds to the promoter and initiates the assembly of a multiprotein preinitiation complex. Several studies have suggested that binding of TBP to the TATA box is an important regulatory step in transcription initiation in vitro. To d...